Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Hepatic TM6SF2 Is Required for Lipidation of VLDL in a Pre-Golgi Compartment in Mice and Rats

doi: 10.1016/j.jcmgh.2021.12.008

Figure Lengend Snippet: Lipid accumulation in tissues of Tm6sf2 KO and WT rats. ( A ) Liver and jejunal tissue sections from chow-fed male WT and KO rats (n = 4/group, 5–6 wk) after a 4-hour fast were stained with Oil Red O and scanned using a NanoZoomer 2.0-HT, digital slide scanner (DM2000), at 10× and 80× magnification. ( B ) Chow-fed male rats (n = 4/group, 3–4 wk) were fasted for 16 hours and then gavaged with corn oil (10 μL/g body weight). Blood was collected at the indicated times and plasma TG levels were measured ( left ). TG absorption was calculated by the area under the curve ( right ). ( C ) Fecal lipids were extracted from feces of chow-fed female rats (n = 4/group, 13–14 wk) that were collected for 2 days. All experiments were repeated at least once and the results were similar. ( D ) Male WT and Tm6sf2 -/- rats (n = 4/group, 6 wk) were fasted for 4 hours and then given Triton WR-1339 (500 mg/kg) and 200 μCi of [35S] methionine (1175 Ci/mmol) intravenously and blood was collected at the indicated times and plasma TG was measured. Rats were killed after 120 minutes. To detect [35S] labeled-ApoB, 25 μL plasma was processed as described in the Methods section. Membranes were exposed to Phosphor Screen in Fujifilm BAS cassette 2040 (Fujifilm) for 1 week and signals were quantified using a Storm Imager (PharosFX; Bio-Rad). Circles and bars indicate means ± SD. Differences between groups were analyzed using the Student unpaired 2-tailed t test. ∗∗ P < .01, and ∗∗∗∗ P < .0001.

Article Snippet: Membranes were exposed to Phosphor Screen in Fujifilm BAS cassette 2040 (Fujifilm) for 1 week and signals were quantified using a Storm Imager (PharosFX; Bio-Rad).

Techniques: Staining, Labeling